It's no secret that BioShock is a veiled analogy for objectivism - Ayn Rand's crackpot philosophy. For those that don't know, objectivism is to conservatives as communism is to liberals: the far-fringe extrapolation of core ideals, taken to a ridiculous and ultimately untennable extreme. Basically, it exists to give rich dillholes an excuse for being rich dillholes.
Anyway, all the things the residents of Rapture are saying come straight from the horse's mouth. None of it matters: they're just rattling off quotes at random. Little Sister's quote could have been "no man may obtain any values from others by resorting to physical force, and no man may initiate the use of physical force against others. The government acts only as a policeman that protects man's rights; it uses physical force only in retaliation and only against those who initiate its use, such as criminals or foreign invaders," but that would have seemed like a response to Nemo's question, which wasn't the intention. Also, it's way too long.
Anyway, the real goal of this strip was just to put the Big Daddies in the Control Cabin diorama and show off how great they looked. Mission accomplished!
Subject Delta: Reality exists as an objective absolute - facts are facts, independent of man's feelings, wishes, hopes or fears.
Big Sister: Man - every man - is an end in himself, not the means to the ends of others. He must exist for his own sake, neither sacrificing himself to others nor sacrificing others to himself. The pursuit of his own rational self-interest and of his own happiness is the highest moral purpose of his life.
Nemo: Seriously, what the #&%@? Are we going to fight or what?
Little Sister: Reason (the faculty which identifies and integrates the material provided by man's senses) is man's only means of perceiving reality, his only source of knowledge, his only guide to action, and his basic means of survival.